Coding

Part:BBa_K3190108:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


Xenopus laevis lutropin-choriogonadotropic hormone receptor LHCGR CDS tagged with myc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene encoding for the receptor was codon optimised, tagged with myc tag in the C-terminus of the receptor and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae, in order to confirm and characterize the protein expression.


Source

Xenopus laevis

References